Research interest

Dr. Brazhnik' research interests are focused on exploring aspects of genome instability in various human primary tissues as possible causal factors in aging and functional decline. Rather common multiple genome variants (structural variations, indels, single-nucleotide variations) in somatic tissues cause genome mosaics and may lead to aging, functional failure and age-related diseases. So far, there is little information about the possible relationship between cell fate and somatic mutation loads. She has tested and applied various efficient procedures for isolating individual single cells or nuclei from different human primary tissues. Upon collection, each individual cell/nucleus is being subjected to single cell whole genome sequencing to search for unique somatic mutational landscape. Besides other cell types analyzed in our lab, I have successfully applied the new procedures to studying complete somatic mutation profiles in normal human liver as a function of age, including comparison of various liver cell types, e.g. differentiated hepatocytes and adult liver stem cells. More recently she has started analysis of genome instability levels in normal human mammary gland tissue from patients with genetic risk of breast cancer compared to healthy donors.

Publications

1. Brazhnik, K., Sun, S., Alani, O., Kinkhabwala, M., Wolkoff, A. W., Maslov, A. Y., Dong, X. & Vijg, J., Jan 31 2020, In : Science Advances. 6, 5, eaax2659.

2. Bilan, R., Ametzazurra, A., Brazhnik, K., Escorza, S., Fernández, D., Uríbarri, M., Sukhanova, A., Nabiev, I. (2017) Quantum-dot-based suspension microarray for multiplex detection of lung cancer markers: preclinical validation and comparison with the Luminex xMAP® system. Scientific Reports, 7, 44668.

3. Bourguet, E., Brazhnik, K., Sukhanova, A., Moroy, G., Brassart-Pasco, S., Martin, A.-P., Villena, I. Bellon, G, Sapi, J., Nabiev, I. (2016) Design, synthesis, and use of MMP-2 inhibitor-conjugated quantum dots in functional biochemical assays. Bioconjugate Chemistry, 27(4), 1067-81.

4. Sukhanova, A., Nabiev, I., Hafian, H., Pluot, M., Cohen, J. H. M., Millot, J.-M., Bilan, R., Brazhnik, K., Baryshnikova, M., Sokolova, Z., Chames, P., Baty, D. (2015) Single-photon and two-photon tumor imaging and diagnosis using oriented conjugates of single-domain antibodies and quantum dots. Proceedings of International Conference NANOMEETING 2015: Physics, Chemistry and Applications of Nanostructures – Reviews and Short Notes, O-31, 495-498.

5. Bilan, R., Brazhnik, K., Chames, P., Baty, D., Nabiev, I., Sukhanova, A. (2015) Oriented conjugates of single-domain antibodies and fluorescent quantum dots for highly sensitive detection of tumor-associated biomarkers in cells and tissues. Physics Procedia, 73, 228–234.

6. Brazhnik, K., Sokolova, Z., Baryshnikova, M., Bilan, R., Nabiev, I., Sukhanova, A. (2015) Multiplexed analysis of serum breast and ovarian cancer markers by means of suspension bead–quantum dot microarrays. Physics Procedia, 73, 235–240.

7. Brazhnik, K., Sokolova, Z., Baryshnikova, M., Bilan, R., Efimov, A., Nabiev, I., Sukhanova, A. (2015) Quantum dot-based lab-on-a-bead system for multiplexed detection of free and total prostate-specific antigens in clinical human serum samples. Nanomedicine: NBM, 11 (5), 1065-1075.

8. Rousserie, G., Grinevich, R., Brazhnik, K., Even-Desrumeaux, K., Reveil, B., Tabary, T., Chames, P., Baty, D., Cohen, J. H. M., Nabiev, I., Sukhanova, A. (2015) Detection of carcinoembryonic antigen using single-domain or full-size antibodies stained with the quantum dot conjugates. Analytical Biochemistry, 478, 26-32.

9. Krivenkov, V.A., Solovyeva, D.O., Samokhvalov, P.S., Grinevich, R.S., Brazhnik, K.I., Kotkovskii, G.E., Lukashev, E.P., Chistyakov, A.A. (2014) Resonance energy transfer in nano–bio hybrid structures can be modulated by UV laser irradiation. Laser Physics Letters, 11 (11), Article 115601.

10. Krivenkov, V.A., Solovyeva, D.O., Samokhvalov, P.S., Brazhnik, K.I., Kotkovskii, G.E., Lukashev, E.P., Chistyakov, A.A., Nabiev, I.R. (2014) Photoinduced modification of quantum dot optical properties affects bacteriorhodopsin photocycle in a (quantum dot)–bacteriorhodopsin hybrid material. Journal of Physics: Conference Series, 541, Article 012045.

11. Brazhnik, K., Nabiev, I., Sukhanova, A. (2014) Advanced procedure for oriented conjugation of full-size antibodies with quantum dots. In Quantum Dots: Applications in Biology, Methods in Molecular Biology (Fontes, A. and Santos, B. S. Eds); Springer Science+Business Media New York, 1199, 55-66.

12. Brazhnik, K., Nabiev, I., Sukhanova, A. (2014) Oriented conjugation of single-domain antibodies and quantum dots. In Quantum Dots: Applications in Biology, Methods in Molecular Biology (Fontes, A. and Santos, B. S. Eds); Springer Science+Business Media New York, 1199, 129-140.

13. Brazhnik, K., Grinevich, R., Efimov, A., Nabiev, I., Sukhanova, A. (2014) Development and potential applications of microarrays based on fluorescent nanocrystal-encoded beads for multiplexed cancer diagnostics. Proceedings of SPIE, Biophotonics: Photonic Solutions for Better Health Care IV, 9129, Article 91292C.

14. Shebzukhov, Y. V., Horn, K., Brazhnik, K. I., Drutskaya, M. S., Kuchmiy, A. A., Kuprash, D. V., and Nedospasov, S. A. (2014) Dynamic changes in chromatin conformation at the TNF transcription start site in T helper lymphocyte subsets, European journal of immunology 44, 251-264.